Mastering HPLC Troubleshooting Basics: A Complete Guide with HumanoForte

By HumanoForte Team, Subject Matter Expert

High-Performance Liquid Chromatography (HPLC) is a cornerstone analytical technique in the pharmaceutical industry, used for everything from raw material testing to stability studies and finished product release. Yet, even experienced analysts encounter issues such as baseline noise, tailing peaks, or inconsistent retention times. Mastering HPLC troubleshooting is crucial for ensuring data integrity, regulatory compliance, and efficient lab operations. HumanoForte supports analytical professionals by offering a wealth of resources: expert-written troubleshooting guides, video tutorials, and a vibrant community forum where you can post your chromatograms and get real-time feedback from seasoned analysts. Our job portal also features specialized listings for analytical chemists and method development experts, helping you advance your career in this high-demand field. Whether you’re just starting out or aiming to become a lab supervisor, HumanoForte provides the knowledge, connections, and opportunities you need to excel at HPLC and beyond.

Common HPLC Issues and Solutions

• Baseline Noise: Check mobile phase quality, degas solvents, and verify detector stability.
• Tailing Peaks: Inspect column integrity, replace frits, and ensure proper sample preparation.
• Ghost Peaks: Clean injector and sample loop, and use fresh solvents.
• Variable Retention Times: Monitor flow rate accuracy and column temperature consistency.
• Low Sensitivity: Confirm detector settings and sample concentration.

How HumanoForte Helps You Troubleshoot

• Access to troubleshooting checklists and quick-reference guides
• Community forum for sharing chromatograms and getting expert advice
• Webinars and video tutorials on advanced HPLC techniques
• Job listings for analytical and quality control roles

Further Reading

• Essential Certifications for Pharma QA
• GMP Audit Preparation Checklist
• Explore Analytical Chemistry Jobs on HumanoForte

Conclusion

With HumanoForte’s expert resources, community support, and targeted job listings, you can become an HPLC troubleshooting pro—boosting your value in the lab and advancing your pharma career.